General talk through my background and about the role What level of experience I have with flow cytometry, (very specific questions about the technique) max number of colours in a panel I have used, how do I decide between fluorochromes for different markers, how do I design panels, how do you gate on positive cells, if I have any experience with assay validation, what to do if the fluorescence was not as expected, what do you do if a marker was not expressed, how to set up a multi-colour panel, what controls do you use
